ISSN 1306-0015 | E-ISSN 1308-6278
Original Article
Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy
1 Pediatric Research Center, Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy  
2 Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy  
3 Department of Pediatrics. Scientific Institute “Casa Sollievo della Sofferenza”, University of Foggia, Italy; European Paediatric Association/Union of National European Paediatric Societies and Associations, Berlin, Germany.  
Turk Pediatri Ars 2017; 52: 208-212
DOI: 10.5152/TurkPediatriArs.2017.6290
Key Words: Challenge, cow milk, cytokines, immunoglobulin, interleukin, tumor necrosis factor
Abstract

Aim: The aim of the study was to investigate the level of cytokines in cultures of cow’s milk protein- stimulated peripheral blood mononuclear cells of patients with cow’s milk protein allergy.

 

Material and Methods: Eleven children with cow’s milk protein allergy and 11 non-allergic controls were studied. Their peripheral blood mononuclear cells were cultured alone and in the presence of cow’s milk α-lactalbumin; β-lactoglobulin; αS 1, αS 2, β, and κ-casein fraction mixtures; and a cow’s protein mixture from whole milk. Production of cytokines, tumor necrosis factor-α, interleukin-10, and interleukin-12 were determined in culture supernatants.

 

Results: In cow’s milk protein-stimulated peripheral blood mononuclear cell cultures of children with cow’s milk protein allergy, tumor necrosis factor-α, interleukin-10, and interleukin-12 production was significantly higher than in non-allergic controls (p<0.05). No difference in cytokine production was found between cultures obtained from unstimulated peripheral blood mononuclear cell cultures of both cow’s milk protein allergy and non-allergic controls.

 

 

Conclusions: The findings of this preliminary study align with data from the literature suggesting that the investigation of tumor necrosis factor-α, interleukin-10, and interleukin-12 in cow’s milk protein-stimulated peripheral blood mononuclear cell cultures of children may be taken in further consideration to explore whether they might have a predictive role for cow’s milk protein allergy. Further studies are therefore needed to extensively investigate this issue.

 

Cite this article as: D’Apolito F, Campanozzi A, Giardino I, Pettoello-Mantovani M. Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy. Turk Pediatri Ars 2017; 52: 208-12.

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